A XENOGENIC IMMUNE RESPONSE TOWARDS STZ-RINM5F CELLS REVEALS CYTOKERATIN18 AS A NOVEL IMMUNOGENIC ANTIGEN THAT MAY PREDISPOSE TOWARDS TYPE-1 DIABETES
نویسندگان
چکیده
Studies suggest certain stimuli like apoptosis behind the aberrant expression of circulating specific auto-antibodies in Type-1 diabetic patients. In present study, we investigated whether Streptozotocin (a diabetogenic compound) induced Rat RINm5f Insulinoma cells can expose immunogenic cryptic antigens. A time course treatment with 4 mM revealed 6hrs as minimal period to induce without much effect on viability.Subsequent immunization mice untreated and treated highly reactive sera from 6hr STZ cells. Hybridoma technique showed a clone named sup160which secretedIgG1 type monoclonal antibody.Parallely, show that STZ-RINm5f challenged displayed both humoral cellular immune response, shown by increased presence IgG1 / IgG2a subclass antibodies overexpression IFNγ, IL-4 TNFα splenocytes. FACS confocal imaging further established reactivity sup160 antibody cell surface antigen. The antigen was identified be cytokeratin18 protein detected 2D gel electrophoresis mass spectrometry. conclusion, have successfully novel xenogenic model system for identification new auto-antigen diabetes, although studies are warranted human subjects.
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ژورنال
عنوان ژورنال: International journal of advanced research
سال: 2022
ISSN: ['2707-7802', '2707-7810']
DOI: https://doi.org/10.21474/ijar01/15083